Inhibitory effects of PARP-1 inhibitor on growth of human osteosarcoma cells.

Objective To investigate the influence of PARP-1 inhibitor(3-aminobenzamide,3-AB) on the proliferation and apoptosis of osteosarcoma cells.Methods The U2OS cells were routinely cultured.CCK-8 was used to analyze cell proliferation,apoptosis was assessed by flow cytometry,the caspase-3 activity was measured by caspase-3 colorimetric assay kit,and the expression of Bcl-2 and Bax was detected by using Western blot.Results 3-AB suppressed the proliferation of osteosarcoma cells in a dose-and time-dependent manner.It also increased apoptosis ratio of osteosarcoma cells,after treated with 3-AB,the caspase-3 activity in osteosarcoma cells was elevated,the expression of Bax increased,and the expression of Bcl-2 first slightly increased and then decreased.Conclusion 3-AB suppresses cell growth and induces apoptosis in human osteosarcoma cells.Our study provided a preliminary evidence by using PARP-1 as a new target for osteosarcoma treatment.