Development and Validation of Derivative Spectrophotometric Method for Simultaneous Estimation of Domperidone and Rabeprazole Sodium in Bulk and Dosage Forms

The use of first order derivative spectroscophotometry allowed simultaneous determination of domperidone and rabeprazole sodium, in fixed dose combination products. The absorbance values at 253.2 nm and 266.4 nm of first derivative spectrum was used for the estimation of domperidone and rabeprazole sodium, respectively without mutual interference. This method obeyed beer’s law in the concentration range of 9-45 µg/ml and 6-30 µg/ml for both domperidone and rabeprazole sodium, respectively. The results of analysis have been validated statistically and recovery studies confirmed the accuracy of the proposed method.