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Free Radical Scavenging Activity of Leaf Extracts of Indigofera Aspalathoides - An in vitro Analysis

A. Philips, Sachu Philip, Venkatesan Arul, B Padmakeerthiga, V. C. Renju, S Santha, Subramaniam Sethupathy

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Abstract

The present study was aimed to evaluate the free radical scavenging activity of two fractions from the leaves of Indigofera Aspalathoides. Four invitro models namely- DPPH radical, ABTS radical, Nitric oxide radical and hydroxyl radical scavenging assays were used to analyze the activity. Amount of polyphenolic compounds, major phytochemical with antioxidant activity was also estimated. Both fractions displayed significant antioxidant activity when compared to standard antioxidants. Even though Chloroform fraction contained less amount of polyphenolic compounds, it showed more radical scavenging activity than the Ethanol fraction which indicates the role of structural features of polyphenolic compounds with respect to their Antioxidant potential.

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What this paper is about

The present study was aimed to evaluate the free radical scavenging activity of two fractions from the leaves of Indigofera Aspalathoides. Four invitro models namely- DPPH radical, ABTS radical, Nitric oxide radical and hydroxyl radical scavenging assays were used to analyze the activity. Amount of polyphenolic compounds, major phytochemical with antioxidant activity was also estimated. Both fractions displayed significant antioxidant activity when compared to standard antioxidants. Even though Chloroform fraction contained less amount of polyphenolic compounds, it showed more radical scavenging activity than the Ethanol fraction which indicates the role of structural features of polyphenolic compounds with respect to their Antioxidant potential.

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Available abstract

The present study was aimed to evaluate the free radical scavenging activity of two fractions from the leaves of Indigofera Aspalathoides. Four invitro models namely- DPPH radical, ABTS radical, Nitric oxide radical and hydroxyl radical scavenging assays were used to analyze the activity. Amount of polyphenolic compounds, major phytochemical with antioxidant activity was also estimated. Both fractions displayed significant antioxidant activity when compared to standard antioxidants. Even though Chloroform fraction contained less amount of polyphenolic compounds, it showed more radical scavenging activity than the Ethanol fraction which indicates the role of structural features of polyphenolic compounds with respect to their Antioxidant potential.

Key concepts: Polyphenol, Chemistry, Scavenging, DPPH, Antioxidant, ABTS, Hydroxyl radical, Phytochemical

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