IVITRO ATIOXIDAT ACTIVITY OF AQUEOUS LEAF EXTRACT OF SOLAUMIGRUM LI.

Summary The objective of the present study was to establish in-vitro antioxidant activity of aqueous leaf extract of Solanum nigrum (Family: Solanaceae) in different in-vitro experimental methods like Diphenylpicrylhydrazyl (DPPH) radical, peroxide radical, superoxide radical and nitric oxide radical scavenging activity with reference to standard antioxidant ascorbic acid. The total antioxidant potential and reducing power were also determined. The plant extract shown the total antioxidant activity of 54.16 mg ascorbic acid equivalent/g as compared to 117.83 mg of the reference standard ascorbic acid. The reducing power of the extract was found to significant and in a concentration dependent manner. The test extract shown marked antioxidant activity with an IC 50 value of 165 µg/mL for DPPH radical, 472 µg/mL for superoxide radical, 417 µg/mL for H 2O2 radical and 483 µg/mL for nitric oxide radical. Hence basing on the above results it was concluded that the aqueous extract of leaves of S.nigrum showed significant antioxidant activity.