ESTIMATION OF ANTIOXIDANT POTENTIAL OF AQUEOUS EXTRACT OF FICUS BENGALENSIS LEAF ON GASTRIC ULCER Research Article

In this study, antioxidant activity of aqueous extract of Ficus bengalensis (FBE) leaf extract was investigated for its free radical scavenging activity by adopting various in vitro and in vivo models. The extract was investigated for its antioxidant activity by DPPH radical scavenging activity, hydroxyl radical scavenging activity, reducing capacity, hydrogen peroxide activity, determination of total phenolic content using Folin-Ciocalteu's phenolic reagent showed maximum scavenging of DPPH radical (89.26%) at 250 mg ml -1 concentration and hydrogen peroxide (50.61%) at 1000mg ml -1 concentration, respectively and the IC50 were to be 67.10 and 23.54 µg/ mL, for FREA and ascorbic acid (Std.) Reducing power was also dose dependent and total phenolic content evaluated that 1 mg of FBE contains 5.2µg equivalent of gallic acid. In case of in vivo antioxidant activity, on lipid peroxidation (LPO), P<0.05, superoxide dismutase (SOD), P<0.01, and catalase (CAT), P<0.001 activity were found to be significant at various level in both models.