Development of RAPD markers for authentication of Piper nigrum (L.)

In this study, the RAPD (Random Amplified Polymorphic DNA) technique was employed for authentication of Piper nigrum (black pepper) from its adulterant Carica papaya . Eight decamer oligonucleotide primers were screened in the RAPD analysis for identification of genuine and adulterant samples using the DNA isolated from the dried seed of samples. Out of eight primers used, five gave species-specific reproducible unique amplicons, which could clearly distinguish genuine as well as adulterant samples having similar morphology. RAPD could thus, help to serve as a complementary tool for quality control of genuine samples sold in the local markets.