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2011•AFRICAN JOURNAL OF BIOTECHNOLOGYOpen access

DNA fingerprinting for the authentication of Ruta graveolens

Khan Salim, Kh Kh, Jabeen Mirza a, Zainul Abdin Malik

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Abstract

Ruta graveolens is a small aromatic shrub and has been used medicinally and magically, since ancient times. In this study, random amplified polymorphic DNA (RAPD) was employed to develop reproducible markers for authentication of this species from its adulterant Euphorbia dracunculoides. The random decamer oligonucleotide primers (42) were screened for identification of genuine and adulterant samples using the DNA isolated from the dried leaf, seed and stem of both samples. Out of 42 primers, 10 gave faint band, 12 gave species-specific reproducible unique band and the remaining did not amplify the DNA. RAPD couldthus, serve as a complementary tool for quality control. Key words: Adulterant, Euphorbia dracunculoides, herbal drugs, random amplified polymorphic DNA.

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What this paper is about

Ruta graveolens is a small aromatic shrub and has been used medicinally and magically, since ancient times. In this study, random amplified polymorphic DNA (RAPD) was employed to develop reproducible markers for authentication of this species from its adulterant Euphorbia dracunculoides. The random decamer oligonucleotide primers (42) were screened for identification of genuine and adulterant samples using the DNA isolated from the dried leaf, seed and stem of both samples. Out of 42 primers, 10 gave faint band, 12 gave species-specific reproducible unique band and the remaining did not amplify the DNA. RAPD couldthus, serve as a complementary tool for quality control. Key words: Adulterant, Euphorbia dracunculoides, herbal drugs, random amplified polymorphic DNA.

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Available abstract

Ruta graveolens is a small aromatic shrub and has been used medicinally and magically, since ancient times. In this study, random amplified polymorphic DNA (RAPD) was employed to develop reproducible markers for authentication of this species from its adulterant Euphorbia dracunculoides. The random decamer oligonucleotide primers (42) were screened for identification of genuine and adulterant samples using the DNA isolated from the dried leaf, seed and stem of both samples. Out of 42 primers, 10 gave faint band, 12 gave species-specific reproducible unique band and the remaining did not amplify the DNA. RAPD couldthus, serve as a complementary tool for quality control. Key words: Adulterant, Euphorbia dracunculoides, herbal drugs, random amplified polymorphic DNA.

Key concepts: Adulterant, RAPD, Biology, DNA profiling, Ruta graveolens, Traditional medicine, DNA, Botany

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