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IMMOBILIZATION OF CELLULASE ENZYME IN CALCIUM ALGINATE GEL AND ITS IMMOBILIZED STABILITY

Trần Quốc Việt, Nguyễn Phước Minh, Dong Thi, Anh Dao

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Abstract

In this study, cellulase enzyme is immobilized in calcium alginate bead by entrapment method. Sodium alginate concentration used for alginate bead forming is 2%. Immobilized enzyme activity is evaluated on carboxymethyl cellulose (CMC). The maximum efficiency of enzyme immobilization is 83.645% with immobilized time 30 minute and bead diameter 3mm. The optimum pH value of immobilized enzyme and free enzyme is 4.5. The optimum temperature value of immobilized enzyme is higher than free enzyme, 60 o C and 55 o C respectively. Immobilized enzyme is more stable versus the change of pH and temperature of environment than free enzyme. Immobilized enzyme can stand in higher acidity and temperature than free enzyme. Immobilized cellulase could be reused many times. Immobilized enzyme activity remains 69.2% after 5 recycles and still 20.3% after 8 recycles.

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What this paper is about

In this study, cellulase enzyme is immobilized in calcium alginate bead by entrapment method. Sodium alginate concentration used for alginate bead forming is 2%. Immobilized enzyme activity is evaluated on carboxymethyl cellulose (CMC). The maximum efficiency of enzyme immobilization is 83.645% with immobilized time 30 minute and bead diameter 3mm. The optimum pH value of immobilized enzyme and free enzyme is 4.5. The optimum temperature value of immobilized enzyme is higher than free enzyme, 60 o C and 55 o C respectively. Immobilized enzyme is more stable versus the change of pH and temperature of environment than free enzyme. Immobilized enzyme can stand in higher acidity and temperature than free enzyme. Immobilized cellulase could be reused many times. Immobilized enzyme activity remains 69.2% after 5 recycles and still 20.3% after 8 recycles.

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Available abstract

In this study, cellulase enzyme is immobilized in calcium alginate bead by entrapment method. Sodium alginate concentration used for alginate bead forming is 2%. Immobilized enzyme activity is evaluated on carboxymethyl cellulose (CMC). The maximum efficiency of enzyme immobilization is 83.645% with immobilized time 30 minute and bead diameter 3mm. The optimum pH value of immobilized enzyme and free enzyme is 4.5. The optimum temperature value of immobilized enzyme is higher than free enzyme, 60 o C and 55 o C respectively. Immobilized enzyme is more stable versus the change of pH and temperature of environment than free enzyme. Immobilized enzyme can stand in higher acidity and temperature than free enzyme. Immobilized cellulase could be reused many times. Immobilized enzyme activity remains 69.2% after 5 recycles and still 20.3% after 8 recycles.

Key concepts: Immobilized enzyme, Cellulase, Calcium alginate, Chemistry, Chromatography, Enzyme, Enzyme assay, Carboxymethyl cellulose

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IMMOBILIZATION OF CELLULASE ENZYME IN CALCIUM ALGINATE GEL AND ITS IMMOBILIZED STABILITY — Research Paper | ScholarLens