Construction and screening of phage display single-chain Fv antibody library against UV-attenuated Schistosoma japonicum cercariae.

Objective To construct a high efficient UV-attenuated Schistosoma japonicum cercariae single chain Fv(ScFv)phage display library and screen for the specific ScFv by the potential protective Schistosoma japonicum cercariae antigen 66-68kDa(SCA66-68kDa),one natural molecular antigen,which based on the high protection of both the UV-attenuated cercariae vaccines and natural molecular vaccine.Methods After selected the optimum conditions of ultraviolet irradiation,the BALB / c mice were immunized with UV-attenuated Schistosoma japonicum cercariae,then the serum of these mice was transferred passively to other mice.In order to evaluate the protection potential of UV-attenuated cercariae serum,once we get the protective serum,the spleens of these mice was used to prepare RNA.Then we used the methods of phage display antibody library to construct the single-chain Fv(scFv) antibody library against UV-attenuated Schistosoma japonicum cercariae and characterized it from the way of capacity and diversity.scFv antibody library was screened against UV-attenuated Schistosoma japonicum cercariae with the purified antigen of