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Determination of imperatorin and isoimperatorin in Radix Angelicae dahuricae by HPLC

Minru Jia, Mengyue Wang, Jin Qumo, Yuying Ma, Tang Shengwu, Ying Xiong, Guihua Jiang

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Abstract

OBJECTIVE To establish an HPLC method for determination of imperatorin and isoimperatorin in Radix Angelicae dahuricae.METHODS The method was chromatographed on petruim C 18 column(5 μm,250 mm×4.6 mm) and the mobile phase was methanol-water (70∶30).Imperatorin and isoimperatorin were detected at 254 nm.RESULTS The standard curve was linear within the concentration range of 0.974-3.869 mg imperatorin and 0.360-2.640 mg isoimperatorin,respectively.The precision was 2.85%( n =5).The recovery was 99.59% imperatorin and 98.14% isoimperatorin,respectively.CONCLUSION This method is rapid,reproducible and accurate.It could be used for quality control for Angelila dehurica as a commodity.

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OBJECTIVE To establish an HPLC method for determination of imperatorin and isoimperatorin in Radix Angelicae dahuricae.METHODS The method was chromatographed on petruim C 18 column(5 μm,250 mm×4.6 mm) and the mobile phase was methanol-water (70∶30).Imperatorin and isoimperatorin were detected at 254 nm.RESULTS The standard curve was linear within the concentration range of 0.974-3.869 mg imperatorin and 0.360-2.640 mg isoimperatorin,respectively.The precision was 2.85%( n =5).The recovery was 99.59% imperatorin and 98.14% isoimperatorin,respectively.CONCLUSION This method is rapid,reproducible and accurate.It could be used for quality control for Angelila dehurica as a commodity.

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Available abstract

OBJECTIVE To establish an HPLC method for determination of imperatorin and isoimperatorin in Radix Angelicae dahuricae.METHODS The method was chromatographed on petruim C 18 column(5 μm,250 mm×4.6 mm) and the mobile phase was methanol-water (70∶30).Imperatorin and isoimperatorin were detected at 254 nm.RESULTS The standard curve was linear within the concentration range of 0.974-3.869 mg imperatorin and 0.360-2.640 mg isoimperatorin,respectively.The precision was 2.85%( n =5).The recovery was 99.59% imperatorin and 98.14% isoimperatorin,respectively.CONCLUSION This method is rapid,reproducible and accurate.It could be used for quality control for Angelila dehurica as a commodity.

Key concepts: Imperatorin, Chemistry, Radix (gastropod), Chromatography, High-performance liquid chromatography, Botany, Biology

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Determination of imperatorin and isoimperatorin in Radix Angelicae dahuricae by HPLC — Research Paper | ScholarLens