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ANTIOXIDANT ACTIVITY OF RIPE PEPINO FRUIT (SOLANUM MURICATUM AITON)

Sudha Govindan, M. Sangeetha Priya, R. Indhu Shree, S. Vadivukkarasi

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Abstract

The aim of the study was to evaluate the antioxidant activity, phenol and flavonoid content of ethyl acetate extract of ripe pepino fruit.  The antioxidant activities were examined by seven different methods, DPPH, reducing power, iron chelation, ABTS, OH, phosphomolybdenum,  and FRAP assays.  In all the methods the extract exhibited good scavenging activity. The EC50 values of ripe ethyl acetate extract on DPPH radical, reducing power, ferrous ion chelation, ABTS radical, FRAP and hydroxyl radical were obtained to be 0.16, 0.82, 39.51, 9.53, 1.06 and  0.26 mg/ml, respectively. Considerable amount of phenol and flavonoids was noticed. This study suggests that possible mechanism of the biological activities may be due to free radical scavenging and antioxidant characteristics which may be due to the presence of polyphenols in the fruit extracts.

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What this paper is about

The aim of the study was to evaluate the antioxidant activity, phenol and flavonoid content of ethyl acetate extract of ripe pepino fruit.  The antioxidant activities were examined by seven different methods, DPPH, reducing power, iron chelation, ABTS, OH, phosphomolybdenum,  and FRAP assays.  In all the methods the extract exhibited good scavenging activity. The EC50 values of ripe ethyl acetate extract on DPPH radical, reducing power, ferrous ion chelation, ABTS radical, FRAP and hydroxyl radical were obtained to be 0.16, 0.82, 39.51, 9.53, 1.06 and  0.26 mg/ml, respectively. Considerable amount of phenol and flavonoids was noticed. This study suggests that possible mechanism of the biological activities may be due to free radical scavenging and antioxidant characteristics which may be due to the presence of polyphenols in the fruit extracts.

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Available abstract

The aim of the study was to evaluate the antioxidant activity, phenol and flavonoid content of ethyl acetate extract of ripe pepino fruit.  The antioxidant activities were examined by seven different methods, DPPH, reducing power, iron chelation, ABTS, OH, phosphomolybdenum,  and FRAP assays.  In all the methods the extract exhibited good scavenging activity. The EC50 values of ripe ethyl acetate extract on DPPH radical, reducing power, ferrous ion chelation, ABTS radical, FRAP and hydroxyl radical were obtained to be 0.16, 0.82, 39.51, 9.53, 1.06 and  0.26 mg/ml, respectively. Considerable amount of phenol and flavonoids was noticed. This study suggests that possible mechanism of the biological activities may be due to free radical scavenging and antioxidant characteristics which may be due to the presence of polyphenols in the fruit extracts.

Key concepts: Antioxidant, Solanum, Botany, Biology, Horticulture, Chemistry, Biochemistry

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