2010•Zhongguo shouyi xuebaoRequires access
The innate immune responses of bovine mammary epithelial cells stimulated by lipopolysaccharide.
Wang Heng, Meng Qi Xia, Qiu ChangWei, Chong Ma, Jianji Li, QI Chang-ming
Open publisher page 0 citations
Abstract
The object of the paper was to study the innate immune responses of bovine mammary epithelial cells stimulated by lipopolysaccharide.Primary bovine mammary epithelial cells were divided from mammary tissue taken from Holstein cows and purified.Different concentration of LPS were co-cultured with BMEC.MTT assay was applied to demonstrate the cell growth process.At 0,2,6 h after co-culture,mRNA level of TLR4,TLR2,CD14,MD-2 were determined by semi-quantitative PCR.High concentration of LPS (100 mg/L) could depress the cell growth significantly.The increase of TLR4,CD14,MD-2 mRNA expression on BMEC were induced by LPS obviously,while no significant increase was detected in TLR2 mRNA expression.The results show that TLR4,CD14,MD-2 play an important role in the recognization procedure of LPS.Innate immune responses of BMEC could be stimulated by LPS.