Antioxidant studies on the ethanolic extract of Zornia gibbosa

Summary The ethanolic extract of Zornia zibbosa, is widely used in the indigenous system of medicine was studied for its in vitro scavenging activity in different methods viz. DPPH, ABTS radical scavenging, lipid peroxidation, iron chelation activity, superoxide scavenging, total antioxidant capacity and antioxidant haemoglobin glycosylation. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 14.78µg/ml for lipid peroxidation, 40.29 µg/ml for ABTS scavenging, 83.11µg/ml for iron chelating activity, 105.90µg/ml for DPPH scavenging and 97.96µg/ml for superoxide scavenging. Total antioxidant capacity was found to be 17.96 µg/ml. In Haemaglobin glycosylation model the % scavenging was found to be 45.49% and 74.68% at the concentration 0.5 mg/ml and 1.0 mg/ml respectively. In all the methods the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Z. Gibbosa has significant antioxidant activity.