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A Radiolabeled Assay for Aldose Reductase

Ian S. Maddox

Open publisher page 2 citations

Abstract

A radiolabeled assay of aldose reductase has been developed based on the use of 14C-glyceraldehyde as substrate. The reaction is measured by the formation of 14C-glycerol, separation being effected by extraction with organic solvent. The Km value for glyceraldehyde has been estimated as 5.7 × 10−6 M. Inhibition by 2-oxoglutarate has been shown to be noncompetitive with a Ki value of 12.5 mM.

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What this paper is about

A radiolabeled assay of aldose reductase has been developed based on the use of 14C-glyceraldehyde as substrate. The reaction is measured by the formation of 14C-glycerol, separation being effected by extraction with organic solvent. The Km value for glyceraldehyde has been estimated as 5.7 × 10−6 M. Inhibition by 2-oxoglutarate has been shown to be noncompetitive with a Ki value of 12.5 mM.

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Available abstract

A radiolabeled assay of aldose reductase has been developed based on the use of 14C-glyceraldehyde as substrate. The reaction is measured by the formation of 14C-glycerol, separation being effected by extraction with organic solvent. The Km value for glyceraldehyde has been estimated as 5.7 × 10−6 M. Inhibition by 2-oxoglutarate has been shown to be noncompetitive with a Ki value of 12.5 mM.

Key concepts: Aldose reductase, Chemistry, Glyceraldehyde, Aldose, Substrate (aquarium), Glycerol, Chromatography, Aldose reductase inhibitor

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