ScholarLens Back to search
2011•AFRICAN JOURNAL OF BIOTECHNOLOGYOpen access

In vitro plant regeneration in rough lemon (Citrus jambhiri Lush) by direct organogenesis

H. S. Rattanpal, Gag, Kaur eep, Gupta Monika

Open full text 17 citations

Abstract

This study was aimed to optimize the organogenesis in rough lemon (Citrus jambhiri Lush), an important citrus rootstock of India. Organogenesis was induced in epicotyl segments of rough lemon seedlings. Three important factors influencing organogenesis in vitro viz. hormone combination, cut modes and photoperiod were evaluated. Etiolation of seedlings had a positive effect on bud formation and higher number of buds per explant was obtained from etiolated seedlings. Among the cut modes, transverse cut performed better for its effect on the number of regenerated buds and shoots per explant. The best response regarding the number of adventitious buds formed per explant was observed with BAP at the concentration of 2.0 mg/l along with malt extract (500 ppm) and additional dose of 25 g/l sucrose. Key words: Citrus, epicotyl, hypocotyl, etiolation, transverse cut, longitudinal cut, photoperiod.

Open-access reader

About this research paper

What this paper is about

This study was aimed to optimize the organogenesis in rough lemon (Citrus jambhiri Lush), an important citrus rootstock of India. Organogenesis was induced in epicotyl segments of rough lemon seedlings. Three important factors influencing organogenesis in vitro viz. hormone combination, cut modes and photoperiod were evaluated. Etiolation of seedlings had a positive effect on bud formation and higher number of buds per explant was obtained from etiolated seedlings. Among the cut modes, transverse cut performed better for its effect on the number of regenerated buds and shoots per explant. The best response regarding the number of adventitious buds formed per explant was observed with BAP at the concentration of 2.0 mg/l along with malt extract (500 ppm) and additional dose of 25 g/l sucrose. Key words: Citrus, epicotyl, hypocotyl, etiolation, transverse cut, longitudinal cut, photoperiod.

Why it matters

OpenAlex reports 17 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

This study was aimed to optimize the organogenesis in rough lemon (Citrus jambhiri Lush), an important citrus rootstock of India. Organogenesis was induced in epicotyl segments of rough lemon seedlings. Three important factors influencing organogenesis in vitro viz. hormone combination, cut modes and photoperiod were evaluated. Etiolation of seedlings had a positive effect on bud formation and higher number of buds per explant was obtained from etiolated seedlings. Among the cut modes, transverse cut performed better for its effect on the number of regenerated buds and shoots per explant. The best response regarding the number of adventitious buds formed per explant was observed with BAP at the concentration of 2.0 mg/l along with malt extract (500 ppm) and additional dose of 25 g/l sucrose. Key words: Citrus, epicotyl, hypocotyl, etiolation, transverse cut, longitudinal cut, photoperiod.

Key concepts: Epicotyl, Rough lemon, Organogenesis, Etiolation, Rootstock, Explant culture, Shoot, Biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
In vitro plant regeneration in rough lemon (Citrus jambhiri Lush) by direct organogenesis — Research Paper | ScholarLens