2015Global Journal of Biology, Agriculture & Health SciencesRequires access

Direct Organogenesis Of Hypocotyl Explants From In Vitro Seedlings Of Cucumis Anguria L.

J. Jerome Jeyakumar, M. Kamaraj

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Abstract

The Plant growth regulator BAP along with KIN, 2, 4 – D and IBA combination of hormones 1.0 + 0.5 to 4.0 + 2.0 mg/l induced shoots ranging from direct organogenesis of hypocotyl explants from in vitro seedlings of Cucumis anguria L. The length of the shoot ranges from 3.62 to 4.70 cm. When BAP along with IBA was tested for shoot initiation and elongation, remarkable results were observed with a mean shoot length of 7.2 cm. In this experiment it was found that BAP with IBA was suitable for multiple shoot initiation and elongation of shoots with direct organogenesis of hypocotyl explants of C. anguria .

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What this paper is about

The Plant growth regulator BAP along with KIN, 2, 4 – D and IBA combination of hormones 1.0 + 0.5 to 4.0 + 2.0 mg/l induced shoots ranging from direct organogenesis of hypocotyl explants from in vitro seedlings of Cucumis anguria L. The length of the shoot ranges from 3.62 to 4.70 cm. When BAP along with IBA was tested for shoot initiation and elongation, remarkable results were observed with a mean shoot length of 7.2 cm. In this experiment it was found that BAP with IBA was suitable for multiple shoot initiation and elongation of shoots with direct organogenesis of hypocotyl explants of C. anguria .

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Available abstract

The Plant growth regulator BAP along with KIN, 2, 4 – D and IBA combination of hormones 1.0 + 0.5 to 4.0 + 2.0 mg/l induced shoots ranging from direct organogenesis of hypocotyl explants from in vitro seedlings of Cucumis anguria L. The length of the shoot ranges from 3.62 to 4.70 cm. When BAP along with IBA was tested for shoot initiation and elongation, remarkable results were observed with a mean shoot length of 7.2 cm. In this experiment it was found that BAP with IBA was suitable for multiple shoot initiation and elongation of shoots with direct organogenesis of hypocotyl explants of C. anguria .

Key concepts: Hypocotyl, Organogenesis, Cucumis, Shoot, Explant culture, Biology, Botany, Elongation

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