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2000•Infection and ImmunityOpen access

Lipopolysaccharides ofBrucella abortusandBrucella melitensisInduce Nitric Oxide Synthesis in Rat Peritoneal Macrophages

Luis López-Urrutia, Andrés Alonso, Marı́a Luisa Nieto, Yolanda Bayón, Antonio Orduña, Mariano Sánchez Crespo

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Abstract

Smooth lipopolysaccharide (S-LPS) and lipid A of Brucella abortus and Brucella melitensis induced the production of nitric oxide (NO) by rat adherent peritoneal cells, but they induced lower levels of production of NO than Escherichia coli LPS. The participation of the inducible isoform of NO synthase (iNOS) was confirmed by the finding of an increased expression of both iNOS mRNA and iNOS protein. These observations might help to explain (i) the acute outcome of Brucella infection in rodents, (ii) the low frequency of septic shock in human brucellosis, and (iii) the prolonged intracellular survival of Brucella in humans.

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What this paper is about

Smooth lipopolysaccharide (S-LPS) and lipid A of Brucella abortus and Brucella melitensis induced the production of nitric oxide (NO) by rat adherent peritoneal cells, but they induced lower levels of production of NO than Escherichia coli LPS. The participation of the inducible isoform of NO synthase (iNOS) was confirmed by the finding of an increased expression of both iNOS mRNA and iNOS protein. These observations might help to explain (i) the acute outcome of Brucella infection in rodents, (ii) the low frequency of septic shock in human brucellosis, and (iii) the prolonged intracellular survival of Brucella in humans.

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Available abstract

Smooth lipopolysaccharide (S-LPS) and lipid A of Brucella abortus and Brucella melitensis induced the production of nitric oxide (NO) by rat adherent peritoneal cells, but they induced lower levels of production of NO than Escherichia coli LPS. The participation of the inducible isoform of NO synthase (iNOS) was confirmed by the finding of an increased expression of both iNOS mRNA and iNOS protein. These observations might help to explain (i) the acute outcome of Brucella infection in rodents, (ii) the low frequency of septic shock in human brucellosis, and (iii) the prolonged intracellular survival of Brucella in humans.

Key concepts: Brucella melitensis, Brucella, Lipopolysaccharide, Microbiology, Biology, Brucellaceae, Nitric oxide, Brucellosis

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Lipopolysaccharides ofBrucella abortusandBrucella melitensisInduce Nitric Oxide Synthesis in Rat Peritoneal Macrophages — Research Paper | ScholarLens