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2013•Advance Journal of Food Science and TechnologyOpen access

Effect of Grape Procyanidins on Vascular Endothelial Cell Apoptosis by Flow Cytometry Analysis

Peilong Xu, Yipeng Su, Na Na Na

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Abstract

This thesis studied the impact of GPC with different concentrations over the vascular endothelial cells apoptosis by flow cytometry and analyzed the influence of GPC on oxygen free radicals in initiating cell apoptosis. Make use of flow cytometry instrument to detect the apoptosis rate of vascular endothelial cell by adding different dose of GPC separately. Through experiments, the apoptosis rates of normal control group, positive control group, GPC (fifty) group, GPC (one hundred) group, GPC (one hundred and fifty) group, GPC (two hundred) group, separately were 11.89, 12.63, 10.17, 5.45, 4.79 and 4.53%, respectively. The experiment results indicated that GPC possessed distinct inhibitory effect on vascular endothelial cells apoptosis evoked by hydroxyl radical.

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What this paper is about

This thesis studied the impact of GPC with different concentrations over the vascular endothelial cells apoptosis by flow cytometry and analyzed the influence of GPC on oxygen free radicals in initiating cell apoptosis. Make use of flow cytometry instrument to detect the apoptosis rate of vascular endothelial cell by adding different dose of GPC separately. Through experiments, the apoptosis rates of normal control group, positive control group, GPC (fifty) group, GPC (one hundred) group, GPC (one hundred and fifty) group, GPC (two hundred) group, separately were 11.89, 12.63, 10.17, 5.45, 4.79 and 4.53%, respectively. The experiment results indicated that GPC possessed distinct inhibitory effect on vascular endothelial cells apoptosis evoked by hydroxyl radical.

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Available abstract

This thesis studied the impact of GPC with different concentrations over the vascular endothelial cells apoptosis by flow cytometry and analyzed the influence of GPC on oxygen free radicals in initiating cell apoptosis. Make use of flow cytometry instrument to detect the apoptosis rate of vascular endothelial cell by adding different dose of GPC separately. Through experiments, the apoptosis rates of normal control group, positive control group, GPC (fifty) group, GPC (one hundred) group, GPC (one hundred and fifty) group, GPC (two hundred) group, separately were 11.89, 12.63, 10.17, 5.45, 4.79 and 4.53%, respectively. The experiment results indicated that GPC possessed distinct inhibitory effect on vascular endothelial cells apoptosis evoked by hydroxyl radical.

Key concepts: Apoptosis, Flow cytometry, Chemistry, Cell, Molecular biology, Reactive oxygen species, Endothelial stem cell, Radical

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Effect of Grape Procyanidins on Vascular Endothelial Cell Apoptosis by Flow Cytometry Analysis — Research Paper | ScholarLens