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DETECTION OF CYTOLETHAL DISTENDING TOXIN GENES IN SHIGA TOXIN-PRODUCING ESCHERICHIA COLI ISOLATED FROM DIFFERENT SOURCES

Jacek Osek

Open publisher page 5 citations

Abstract

In the present study the cdt gene was detected, using PCR, in 16 out of 65 (24.6%) Shiga toxin-producing E. coli (STEC) strains of different serotypes, isolated from humans, food, cattle, and pigs. Most of the cdt-positive isolates had the cdt-V gene variant (13 strains). All these strains were of O157:NM serotype, possessed the stx2 Shiga toxin and eaeA intimin genes, and were isolated from humans with haemolytic uraemic syndrome (HUS). Only two STEC strains recovered from cattle with diarrhoea had the CDT toxin marker.

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What this paper is about

In the present study the cdt gene was detected, using PCR, in 16 out of 65 (24.6%) Shiga toxin-producing E. coli (STEC) strains of different serotypes, isolated from humans, food, cattle, and pigs. Most of the cdt-positive isolates had the cdt-V gene variant (13 strains). All these strains were of O157:NM serotype, possessed the stx2 Shiga toxin and eaeA intimin genes, and were isolated from humans with haemolytic uraemic syndrome (HUS). Only two STEC strains recovered from cattle with diarrhoea had the CDT toxin marker.

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OpenAlex reports 5 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

In the present study the cdt gene was detected, using PCR, in 16 out of 65 (24.6%) Shiga toxin-producing E. coli (STEC) strains of different serotypes, isolated from humans, food, cattle, and pigs. Most of the cdt-positive isolates had the cdt-V gene variant (13 strains). All these strains were of O157:NM serotype, possessed the stx2 Shiga toxin and eaeA intimin genes, and were isolated from humans with haemolytic uraemic syndrome (HUS). Only two STEC strains recovered from cattle with diarrhoea had the CDT toxin marker.

Key concepts: Intimin, Shiga toxin, Cytolethal distending toxin, STX2, Serotype, Microbiology, Toxin, Biology

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DETECTION OF CYTOLETHAL DISTENDING TOXIN GENES IN SHIGA TOXIN-PRODUCING ESCHERICHIA COLI ISOLATED FROM DIFFERENT SOURCES — Research Paper | ScholarLens