ScholarLens Back to search
2003•Unpublished venueRequires access

PREVALENCE OF cdt-III GENE ENCODING CYTOLETHAL DISTENDING TOXIN (CDT) AMONG ESCHERICHIA COLI STRAINS ISOLATED FROM PIGS WITH DIARRHEA

Jacek Osek, Marcin Weiner

Open publisher page 1 citations

Abstract

Two hundred eighteen Escherichia coli strains isolated from diarrheic pigs were analyzed for the prevalence of the cdt-III gene encoding cytolethal distending toxin III (CDTIII). The CDT-III primers used in the study flanked the fragment responsible for the production of the cdt-IIIA subunit sequence. The PCR system applied in the study also included the E. coli 16S rRNA gene detection, which was used as an internal control. The multiplex PCR (mPCR) developed showed that only 3 of the 218 (1.4 per cent) isolates harboured the gene for CDT-III toxin. Two of these strains had also the genetic marker for cytotoxin necrotizing factor 2 (CNF2). The results obtained indicate that in Poland CDT-III toxin is not a common virulence marker in E. coli strains isolated from pigs with diarrhea.

About this research paper

What this paper is about

Two hundred eighteen Escherichia coli strains isolated from diarrheic pigs were analyzed for the prevalence of the cdt-III gene encoding cytolethal distending toxin III (CDTIII). The CDT-III primers used in the study flanked the fragment responsible for the production of the cdt-IIIA subunit sequence. The PCR system applied in the study also included the E. coli 16S rRNA gene detection, which was used as an internal control. The multiplex PCR (mPCR) developed showed that only 3 of the 218 (1.4 per cent) isolates harboured the gene for CDT-III toxin. Two of these strains had also the genetic marker for cytotoxin necrotizing factor 2 (CNF2). The results obtained indicate that in Poland CDT-III toxin is not a common virulence marker in E. coli strains isolated from pigs with diarrhea.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Two hundred eighteen Escherichia coli strains isolated from diarrheic pigs were analyzed for the prevalence of the cdt-III gene encoding cytolethal distending toxin III (CDTIII). The CDT-III primers used in the study flanked the fragment responsible for the production of the cdt-IIIA subunit sequence. The PCR system applied in the study also included the E. coli 16S rRNA gene detection, which was used as an internal control. The multiplex PCR (mPCR) developed showed that only 3 of the 218 (1.4 per cent) isolates harboured the gene for CDT-III toxin. Two of these strains had also the genetic marker for cytotoxin necrotizing factor 2 (CNF2). The results obtained indicate that in Poland CDT-III toxin is not a common virulence marker in E. coli strains isolated from pigs with diarrhea.

Key concepts: Cytolethal distending toxin, Toxin, Escherichia coli, Biology, Microbiology, Multiplex polymerase chain reaction, Gene, Polymerase chain reaction

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
PREVALENCE OF cdt-III GENE ENCODING CYTOLETHAL DISTENDING TOXIN (CDT) AMONG ESCHERICHIA COLI STRAINS ISOLATED FROM PIGS WITH DIARRHEA — Research Paper | ScholarLens