2005•Acta Crystallographica Section D Biological CrystallographyOpen access

Instrumentation for synchrotron-radiation macromolecular crystallography

Éric Girard, Pierre Legrand, Olga Roudenko, L. Roussier, Patrick Gourhant, Jonathan Gibelin, Didier Dalle, Majid Ounsy, Andrew Thompson, Olof Svensson, Marie-Odile Cordier, Sophie Robin, René Quiniou, Jean‐Philippe Steyer

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Abstract

X-ray diffraction is an extremely important tool for structure determination of biological macromolecules, to the extent that currently around 85% of Protein Data Bank entries result from X-ray measurements. Many of these structure determinations use synchrotron radiation for data collection. This article aims to give synchrotron users an overview of the functioning of a synchrotron beamline and how the performance of various instruments combines to allow the collection of diffraction data.

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X-ray diffraction is an extremely important tool for structure determination of biological macromolecules, to the extent that currently around 85% of Protein Data Bank entries result from X-ray measurements. Many of these structure determinations use synchrotron radiation for data collection. This article aims to give synchrotron users an overview of the functioning of a synchrotron beamline and how the performance of various instruments combines to allow the collection of diffraction data.

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Available abstract

X-ray diffraction is an extremely important tool for structure determination of biological macromolecules, to the extent that currently around 85% of Protein Data Bank entries result from X-ray measurements. Many of these structure determinations use synchrotron radiation for data collection. This article aims to give synchrotron users an overview of the functioning of a synchrotron beamline and how the performance of various instruments combines to allow the collection of diffraction data.

Key concepts: Beamline, Synchrotron radiation, Synchrotron, Diffraction, Instrumentation (computer programming), Optics, X-ray crystallography, Data collection

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