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2004•Journal of Clinical MicrobiologyOpen access

Real-Time Multiplex PCR Assay for Detection of Brucella spp., B. abortus , and B. melitensis

William S. Probert, Kimmi N. Schrader, Nhi Y. Khuong, Susan L. Bystrom, Margot Graves

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Abstract

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

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What this paper is about

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

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Available abstract

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

Key concepts: Brucella, Brucella melitensis, Multiplex, Brucella abortus, Brucellaceae, Multiplex polymerase chain reaction, Brucellosis, Identification (biology)

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