Micropropagation Studies in Jamun (Syzygium Cuminii L.)
Nasim Choudhri, G. S. K. Swamy, R. C. Jagadeesha, M. L. Chavan, Anand B. Mastiholi, G. Prabhuling, H.R. Basavarajappa
Abstract
Nasim Choudhri, G. S. K. Swamy, R. C. Jagadeesha, M. L. Chavan, Anand B. Mastiholi, G. Prabhuling, H.R. Basavarajappa
Abstract
In vitro propagation of the jamun (Syzygium cuminii L.) can be achieved by using single nodal explants taken from the seedlings. Single nodal explants cultured on half strength MS along with 2mg/l BAP + 3% sucrose + 3% activated charcoal was proved to be better for shoot proliferation. The shootlets were successfully rooted on 1/4th strength MS along with 3% sucrose and 2.5 mg/l IBA. The rooted shootlets were then hardened on vermiculite (32.5 % survival) for six weeks and then planted in the polybags and transferred to the field.
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In vitro propagation of the jamun (Syzygium cuminii L.) can be achieved by using single nodal explants taken from the seedlings. Single nodal explants cultured on half strength MS along with 2mg/l BAP + 3% sucrose + 3% activated charcoal was proved to be better for shoot proliferation. The shootlets were successfully rooted on 1/4th strength MS along with 3% sucrose and 2.5 mg/l IBA. The rooted shootlets were then hardened on vermiculite (32.5 % survival) for six weeks and then planted in the polybags and transferred to the field.
Key concepts: Explant culture, Vermiculite, Micropropagation, Activated charcoal, Sucrose, Shoot, Syzygium, Botany