In Vitro Rapid Multiplication of Wrightia Tomentosa (ROXB.) Roem. and Schultz an Endangered Medicinal Tree Species

The protocol for in vitro multiple shoot development and plantlet formation was developed using cotyledonary node and shoot tip explant in Wrightia tomentosa (Roxb.) Roem. and Schultz an endangered medicinally important forest tree. The explants were cultured on ¼ strength MS medium supplemented with various concentrations of BAP. Multiple shoots were induced from the cotyledonary nodal segments and shoot tip explants in all the concentrations of BAP. More percentage of multiple shoot induction was observed in cotyledonary nodal explants compared to shoot tips. Maximum number of shoots proliferation (12±1.07) with high frequency of shooting response was found in shoot tip explants followed by nodal explants at 3.0 mg/L BAP. The micro-shoots were cultured on ¼ strength MS medium with various concentrations of auxins IAA/IBA for in vitro rooting. Profuse rhizogenesis (16.2±2.01, 12.8±1.29 cm) was observed on ¼ strength MS medium supplemented with 1.0 mg/L IAA/1.5 mg/L IBA. The in vitro rooted plantlets were transferred to polycups containing sterile soil: vermiculite (1:1) and hardened in the culture room. These acclimatized plants were transferred into field and the survival percentage was found to be 75. These in vitro regenerated plants were similar to donor plant.