2013•Asian Pacific Journal of Tropical MedicineOpen access
A duplex PCR for rapid and simultaneous detection of Brucella spp. in human blood samples.
Reza Mirnejad, Mozafar mohamadi, Vahbeh Piranfar, Seied Mojtaba Mortazavi, Reza Kachuei
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Abstract
OBJECTIVE: To design a duplex PCR for rapid and simultaneous detection of Brucella species. in human blood samples. METHODS: Fifty-two peripheral bloods samples were collected from suspicious patients with brucellosis. Following DNA extraction, PCR assay were performed, using three primers that could simultaneously identify and differentiate three major species of pathogenic Brucella in humans and animals. RESULTS: Of the 52 peripheral bloods samples tested, 25 sample (48%) showed positive reactions in PCR. Twelve samples were positive for Brucella abortus 39 (B. abortus 39) (23%), 13 for Brucella melitensis 39 (B. melitensis 39) (25%) and 0 for Brucella ovis 39 (B. ovis 39) (0%). CONCLUSIONS: This work demonstrates that in case where specific primers were utilized, duplex PCR has proved to be a simple, fast, and relatively inexpensive method for simultaneous detection of important species of Brucella in clinical samples.