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Indirect Immunofluorescence of Tissues

Wolfgang Härtig, Jean‐Marc Fritschy

Abstract

Abstract Indirect immunofluorescence is a two‐step procedure whereby unlabelled antibodies are first bound to their target antigen and subsequently detected with fluorochrome‐conjugated secondary antibodies (indirect immunofluorescence). This technique is widely used for histological analysis of tissues in basic and clinical research.

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Key concepts: Immunofluorescence, Indirect immunofluorescence, Antibody, Direct fluorescent antibody, Antigen, Primary and secondary antibodies, Pathology, Chemistry