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Construction of Linkage Map Using RAPD and SSR Markers in Soybean (Glycine max)

Jong-Il Chung, James E. Specht

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Abstract

Linkage maps based on molecular markers are valuable tools in plant breeding and genetic studies. A population of 76 RI lines from the mating of A3733 and PI437.088 was evaluated with Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) markers to create soybean molecular linkage map. 302 RAPD and 21 SSR markers were genetically linked and formed forty linkage groups. These linkage groups spanned a genetic distance of 1,775 cM. The average distance between markers was 5.5 cM.

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Linkage maps based on molecular markers are valuable tools in plant breeding and genetic studies. A population of 76 RI lines from the mating of A3733 and PI437.088 was evaluated with Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) markers to create soybean molecular linkage map. 302 RAPD and 21 SSR markers were genetically linked and formed forty linkage groups. These linkage groups spanned a genetic distance of 1,775 cM. The average distance between markers was 5.5 cM.

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Available abstract

Linkage maps based on molecular markers are valuable tools in plant breeding and genetic studies. A population of 76 RI lines from the mating of A3733 and PI437.088 was evaluated with Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) markers to create soybean molecular linkage map. 302 RAPD and 21 SSR markers were genetically linked and formed forty linkage groups. These linkage groups spanned a genetic distance of 1,775 cM. The average distance between markers was 5.5 cM.

Key concepts: RAPD, Genetics, Genetic linkage, Biology, Linkage (software), Genetic linkage map, Microsatellite, Genetic marker

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