2004•Biomedical ChromatographyOpen access

Metabolic studies on the total phenolic acids from the roots of Salvia miltiorrhiza in rats

Jinlan Zhang, Yun He, Ming Cui, Lie Li, Hai-lan Yu, Guifeng Zhang, De‐an Guo

Abstract

Phenolic acids are the main active constituents of Salvia miltiorrhiza Bunge. The metabolism of total phenolic acids from the roots of Salvia miltiorrhiza in rats was investigated. A sample preparation method combining the solid-phase extraction with liquid-liquid extraction was established to separate metabolites from the biological matrix. HPLC-UV and HPLC-MS methods were employed to analyze the metabolites. Five metabolites (M1-M5) were identified by HPLC-MS analysis and comparison with those of the reference standards. The fi ve metabolites were characterized as danshensu (M1), caffeic acid (M2), ferulic acid (M3), isoferulic acid (M4) and methylized ferulic acid (M5), respectively. The possible metabolic pathway of the phenolic acids is proposed.

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Key concepts: Salvia miltiorrhiza, Chemistry, Ferulic acid, Caffeic acid, Chromatography, High-performance liquid chromatography, Phenolic acid, Extraction (chemistry)