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2014•BioMed Research InternationalOpen access

A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus

Chun-Hsien Tseng, Hsiang‐Jung Tsai, Chung-Ming Chang

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Abstract

INTRODUCTION: The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. METHOD: This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. RESULT AND CONCLUSION: This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.

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INTRODUCTION: The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. METHOD: This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. RESULT AND CONCLUSION: This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.

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Available abstract

INTRODUCTION: The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. METHOD: This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. RESULT AND CONCLUSION: This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.

Key concepts: Subtyping, Neuraminidase, Influenza A virus subtype H5N1, Virology, Hemagglutinin (influenza), Biology, Influenza A virus, Virus

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