2012•Molecular Plant BreedingRequires access

Mutagenic Effects of Maize Inbred Line Irradiated by Heavy Ion 7Li

Peng Geng, Duofang Li, Tian-Guang Cao, Yafei Chen, Chunlei Hu, Li Jin, Xuzhao Wang, Yong Zhan

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Abstract

The dry seeds of maize inbred line Yong 19 were radiated by 7 Li heavy ion with the energy 43MeV and the doses were treated with 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy, respectively. The field data for plant height and ear position of maize inbred line induced by heavy ion from M 1 (mutation generation 1) to M 3 were assessed. The results showed that the plant height of the offsprings derived from maize inbred line Yong 19 had significant variation by comparing with that of the control (CK). Genetic fingerprints of M 4 progenies were analyzed by using random amplified polymorphic DNAs (RAPD) method, the results revealed that  there were different effects on maize genome DNA by different doses. The doses of 10 Gy and 40 Gy could yield up higher optimal selective coefficiencies that were about 0.221 4 and 0.166 4, respectively. Compared with the Control, the genetic similarity (GS) coefficients of the doses in 10 Gy and 40 Gy were less than that of other doses. The maize radiated of 10 Gy and 40 Gy were classified into two different groups, and those of 20 Gy, 30 Gy, 50 Gy were congregated into another group.

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What this paper is about

The dry seeds of maize inbred line Yong 19 were radiated by 7 Li heavy ion with the energy 43MeV and the doses were treated with 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy, respectively. The field data for plant height and ear position of maize inbred line induced by heavy ion from M 1 (mutation generation 1) to M 3 were assessed. The results showed that the plant height of the offsprings derived from maize inbred line Yong 19 had significant variation by comparing with that of the control (CK). Genetic fingerprints of M 4 progenies were analyzed by using random amplified polymorphic DNAs (RAPD) method, the results revealed that  there were different effects on maize genome DNA by different doses. The doses of 10 Gy and 40 Gy could yield up higher optimal selective coefficiencies that were about 0.221 4 and 0.166 4, respectively. Compared with the Control, the genetic similarity (GS) coefficients of the doses in 10 Gy and 40 Gy were less than that of other doses. The maize radiated of 10 Gy and 40 Gy were classified into two different groups, and those of 20 Gy, 30 Gy, 50 Gy were congregated into another group.

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Available abstract

The dry seeds of maize inbred line Yong 19 were radiated by 7 Li heavy ion with the energy 43MeV and the doses were treated with 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy, respectively. The field data for plant height and ear position of maize inbred line induced by heavy ion from M 1 (mutation generation 1) to M 3 were assessed. The results showed that the plant height of the offsprings derived from maize inbred line Yong 19 had significant variation by comparing with that of the control (CK). Genetic fingerprints of M 4 progenies were analyzed by using random amplified polymorphic DNAs (RAPD) method, the results revealed that  there were different effects on maize genome DNA by different doses. The doses of 10 Gy and 40 Gy could yield up higher optimal selective coefficiencies that were about 0.221 4 and 0.166 4, respectively. Compared with the Control, the genetic similarity (GS) coefficients of the doses in 10 Gy and 40 Gy were less than that of other doses. The maize radiated of 10 Gy and 40 Gy were classified into two different groups, and those of 20 Gy, 30 Gy, 50 Gy were congregated into another group.

Key concepts: Biology, Inbred strain, Irradiation, Genetics, Line (geometry), Molecular biology, Radiochemistry, Physics

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