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Simultaneous Radioimmunoassay for Luteinizing Hormone and Prolactin

Marianne K. Steele, Christian F. Deschepper

Abstract

A combined radioimmunoassay (RIA) for the measurement of the anterior pituitary proteins luteinizing hormone (LH) and prolactin (PRL) is described and compared with individual RIAs for these hormones. The standard curves and the sample values for LH and PRL were identical when determined in a combined or in an individual RIA. This technique may prove useful to a number of laboratories where it is desirable to determine levels of more than one hormone in limited sample volumes.

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Key concepts: Radioimmunoassay, Prolactin, Luteinizing hormone, Hormone, Endocrinology, Internal medicine, Gonadotropic cell, Anterior pituitary