2006•In Vitro Cellular & Developmental Biology - AnimalRequires access
A NEW CELL LINE FROM LARVAL FAT BODIES OF THE BOLLWORM, HELICOVERPA ARMIGERA (LEPIDOPTERA: NOCTUIDAE)
Huan Zhang, Yong‐An Zhang, Qilian Qin, Yuzhu Wang, Xuan Li, Lin Miao, Zhenxian Yin, Aijun Zhang, Liangjian Qu, Ding Cui, HUAN ZHANG, YONG-AN ZHANG, QILIAN QIN, YUZHU WANG, XUAN LI, LIN MIAO, ZHENXIAN YIN, AIJUN ZHANG, LIANGJIAN QU, CUI DING
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Abstract
A new cell line, designated IOZCAS-Ha-I, was initiated from the fat body of larvae of Helicoverpa armigera (Lepidoptera: Noctuidae) in TNM-FH medium containing 10% fetal bovine serum. Spherical cells were predominant among the various cell types. The cell line showed a typical lepidopteran chromosome pattern ranging from 58 to 239 chromosomes in the majority of the cells. It was confirmed to have originated from the H. armigera by the DNA amplification- fingerprinting polymerase chain reaction (DAF-PCR) technique. The new cell line was only slightly susceptible to the multiple nucleocapsid nuclear polyhedrosis viruses (NPV) from H. armigera.