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1971•Journal of Applied BacteriologyRequires access

A Novel Selective Method for the Production of Bacillus subtilis var. niger Spores

Celia G. Evans, R. Harris‐Smith

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Abstract

Summary. A method for the recurrent selective production of heat resistant spores of Bacillus subtilis var. niger is described. The method embodies some features of both batch and continuous culture, permitting a daily production of 10 1 volumes of culture containing up to 2·3 × 10 10 spores/ml. These spores are stable in storage at −20° and yet germinate readily, giving rise to easily recognizable, pigmented colonies.

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Summary. A method for the recurrent selective production of heat resistant spores of Bacillus subtilis var. niger is described. The method embodies some features of both batch and continuous culture, permitting a daily production of 10 1 volumes of culture containing up to 2·3 × 10 10 spores/ml. These spores are stable in storage at −20° and yet germinate readily, giving rise to easily recognizable, pigmented colonies.

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Available abstract

Summary. A method for the recurrent selective production of heat resistant spores of Bacillus subtilis var. niger is described. The method embodies some features of both batch and continuous culture, permitting a daily production of 10 1 volumes of culture containing up to 2·3 × 10 10 spores/ml. These spores are stable in storage at −20° and yet germinate readily, giving rise to easily recognizable, pigmented colonies.

Key concepts: Spore, Bacillus subtilis, Germination, Bacillus (shape), Biology, Food science, Microbiology, Chemistry

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