2011American Journal of BotanyRequires access

Development of a novel set of microsatellite markers for Castor bean, Ricinus communis (Euphorbiaceae)1

Miklos Maximiliano Bajay, Maria Imaculada Zucchi, Tammy Aparecida Manabe Kiihl, Carlos Eduardo Araújo Batista, Mariza Monteiro, José Baldin Pinheiro

Open publisher page 25 citations

Abstract

PREMISE OF STUDY: Microsatellite primers were developed for castor bean (Ricinus communis L.) to investigate genetic diversity and population structure, and to provide support to germplasm management. METHODS AND RESULTS: Eleven microsatellite loci were isolated using an enrichment cloning protocol and used to characterize castor bean germplasm from the collection at the Instituto Agronômico de Campinas (IAC). In a survey of 76 castor bean accessions, the investigated loci displayed polymorphism ranging from two to five alleles. CONCLUSIONS: The information derived from microsatellite markers led to significant gains in conserved allelic richness and provides support to the implementation of several molecular breeding strategies for castor bean.

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PREMISE OF STUDY: Microsatellite primers were developed for castor bean (Ricinus communis L.) to investigate genetic diversity and population structure, and to provide support to germplasm management. METHODS AND RESULTS: Eleven microsatellite loci were isolated using an enrichment cloning protocol and used to characterize castor bean germplasm from the collection at the Instituto Agronômico de Campinas (IAC). In a survey of 76 castor bean accessions, the investigated loci displayed polymorphism ranging from two to five alleles. CONCLUSIONS: The information derived from microsatellite markers led to significant gains in conserved allelic richness and provides support to the implementation of several molecular breeding strategies for castor bean.

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Available abstract

PREMISE OF STUDY: Microsatellite primers were developed for castor bean (Ricinus communis L.) to investigate genetic diversity and population structure, and to provide support to germplasm management. METHODS AND RESULTS: Eleven microsatellite loci were isolated using an enrichment cloning protocol and used to characterize castor bean germplasm from the collection at the Instituto Agronômico de Campinas (IAC). In a survey of 76 castor bean accessions, the investigated loci displayed polymorphism ranging from two to five alleles. CONCLUSIONS: The information derived from microsatellite markers led to significant gains in conserved allelic richness and provides support to the implementation of several molecular breeding strategies for castor bean.

Key concepts: Ricinus, Euphorbiaceae, Biology, Castor beans, Botany, Microsatellite, Genetics, Gene

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Development of a novel set of microsatellite markers for Castor bean, Ricinus communis (Euphorbiaceae)1 — Research Paper | ScholarLens