2010•Applied and Environmental MicrobiologyOpen access

Microbial Community Fingerprinting by Differential Display-Denaturing Gradient Gel Electrophoresis

M. Carmen Portillo, D. Villahermosa, Alfonso Corzo, Juan Miguel González Grau

Abstract

Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison.

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Key concepts: Temperature gradient gel electrophoresis, DNA profiling, Fingerprint (computing), Biology, Primer (cosmetics), Gel electrophoresis, Genetics, DNA