2003Journal of Food ScienceRequires access

Comparison of the Real‐time Microrespirometer and Aerobic Plate Count Methods for Determination of Microbial Quality in Ground Beef

X. Li, Yuch‐Ping Hsieh

Open publisher page 3 citations

Abstract

ABSTRACT Real‐time microrespirometer measurements were compared with the aerobic plate count (APC) method to assess microbial quality of ground beef stored at 4°C and 7°C with and without previous freezing. The samples were monitored daily for CO2 evolution rate (CER) using a microrespirometer, APC, and were evaluated for color and odor changes by a sensory panel. The CER was highly correlated with the APC for all storage conditions ( r 2 = 0.787 to 0.952). The onset of meat spoilage was more closely associated with a specific CER value (>25 μL/h/g) than APC. The new method was found to be more accurate in predicting meat spoilage, especially for previously frozen samples.

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What this paper is about

ABSTRACT Real‐time microrespirometer measurements were compared with the aerobic plate count (APC) method to assess microbial quality of ground beef stored at 4°C and 7°C with and without previous freezing. The samples were monitored daily for CO2 evolution rate (CER) using a microrespirometer, APC, and were evaluated for color and odor changes by a sensory panel. The CER was highly correlated with the APC for all storage conditions ( r 2 = 0.787 to 0.952). The onset of meat spoilage was more closely associated with a specific CER value (>25 μL/h/g) than APC. The new method was found to be more accurate in predicting meat spoilage, especially for previously frozen samples.

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Available abstract

ABSTRACT Real‐time microrespirometer measurements were compared with the aerobic plate count (APC) method to assess microbial quality of ground beef stored at 4°C and 7°C with and without previous freezing. The samples were monitored daily for CO2 evolution rate (CER) using a microrespirometer, APC, and were evaluated for color and odor changes by a sensory panel. The CER was highly correlated with the APC for all storage conditions ( r 2 = 0.787 to 0.952). The onset of meat spoilage was more closely associated with a specific CER value (>25 μL/h/g) than APC. The new method was found to be more accurate in predicting meat spoilage, especially for previously frozen samples.

Key concepts: Plate count, Food spoilage, Meat spoilage, Food science, Odor, Chemistry, Biology, Bacteria

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