2010•DOAJ (DOAJ: Directory of Open Access Journals)Open access
Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind.
Kantamaht Kanchanapoom, N. Buntin, Kamnoon Kanchanapoom
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Abstract
A tissue culture system was established from young leaves of in vitro grown seedling of Torenia fournieri Lind. Leafsegments were cultured on Murashige and Skoog medium (MS) medium supplemented with combinations of NAA and BA. Shoot organogenesis was observed in all growth regulators containing medium. High frequency regeneration was obtained from leaves cultured on MS medium supplemented with 0.05 mg/l NAA plus 3 mg/l BA. When individual isolated shoots were transferred to MS medium devoid of growth regulators, complete plantlets were obtained. Adventitious shoots were also regenerated from callus that was derived from leaf explants and a high frequency of shoot organogenesis was found on MSmedium containing 0.1 mg/l NAA plus 3 mg/l BA.