2011•DOAJ (DOAJ: Directory of Open Access Journals)Open access
Assessment of genetic relatedness among okra genotypes [abelmoschus esculentus (l.) Moench] using rapd markers
K Nagre Prakash, Mottaiyan Pitchaimuthu, Kundapura Venkataramana Ravishankar
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Abstract
DNA based RAPD (Randomly Amplification of Polymorphic DNA) markers have been used extensively to study geneticrelationships in number of crop plants. In this study, 44 okra genotypes collected from different parts of India, were selected toassess genetic distinctiveness and relatedness. Total genomic DNA was extracted and subjected to RAPD analysis using 14arbitrary 10 mer primers. The molecular analysis showed that all the fourteen primers used revealed clear distinction between thegenotypes and they generated a total of 104 RAPD bands most of which were polymorphic across accessions (74.03%). Thenumber of bands resolved per amplification was primer dependent and varied from 4 (OPV-07, OPV-08) to 11 (OPD-05) withaverage number of bands per primer was 7.41. RAPD data were used to calculate a Squared Euclidean Distance matrix, andbased on this, cluster analysis was done using minimum variance algorithm. Cluster analysis showed two major groups. Eachsub-group was characterized using morphological and genetic characteristics of the respective genotypes.