KARAKTERISASI XILANASE DARITrichoderma harzianum EMXJ3

Xylanase is an extracellular enzyme that catalyze the hydrolysis of xylan. This enzymes have expanded their use in many industries processing, such as animal feed, food industry, textiles, pulp and paper industry. Xylanases are produced by micro-organisms such as fungi, bacteria and yeast. Trichoderma harzianum EMXJ3 fungi is a free catabolic repression mutant in producing xylanase enzymes which are biotechnology laboratory, FTP, UGM property. Heterogeneous structure of xylan make each micro-organism produced xylanase enzymes with various characteristic, therefore it is very essential to study characteristic xylanase that produced from Trichoderma harzianum EMXJ3. Xylanase produced with Mandels medium contains birchwood xylan as substrate. Purification method used presipitation with ammonium sulphate 40-60% saturation. This characterization consist of investigation the effect of temperature and pH to xylanase activity, stability of xylanase from temperature and pH measure kinetic parameters, and determine xylanase molecular weight. Xylanase can purified with ammonium sulphate 0-40% saturated with 62% recovery of xylanase activity and 2,4 fold purification. Optimum activity of pH and temperature were pH 5 and 50ÂoC respectively. Xylanase was stable at pH 5, retained 98% of its original activity after incubation for 12 h. It was stable at temperature 50ÂoC, retained 97% of it original activity after incubation for 90 min. The Km and Vmax values were 40 mg/ml and 200 µmol ml-1 min-1 respectively. SDS-PAGE of xylanase showed single band with molecular weight of 22 kDa