Expressions of CD151, Integrin alpha3 beta1 and MMP7 in Breast Cancer Tissue Microarray and their Clinicopathological Significance

Purpose: CD151, one of tetraspanins has been suggested to regulate cancer invasion and metastasis. We examined whether it is expressed in primary breast carcinoma and whether has relation with integrina3e1 and MMP7. Methods: By using immunohisto chemical S-P method, the expressions of CD151, integrina3e1 and MMP7 proteins in breast cancer tissue micro array (TMA) were tested. Their correlations with clinicopathological features were analyzed. The relationship among three proteins was also analyzed. Comparison between qualitative data was performed using Chi-square test. Results: The positive rates of CD151, integrina3e1 and MMP7 expressions were 0.0% (0/39) , 7.7%(3/39)and 10.3% (4/39) in normal breast tissue, respectively, 33.3% (10/30) , 30%(9/30) and 40.0% (12/30) in tissues of carcinoma in situ, respectively, 59.0%(59/100), 70.0%(70/100) and 70.0% (70/100) in cancerous tissues, respectively. The differences of three kinds of protein expression rates among the three groups were significant (λ2=41.276, λ2=48.618, λ2=41.801). The expressions of three proteins in breast cancer tissues weren't associated with patient's ages and the histological type (P>;0.05). But they significantly related with differentiated degree of cancer tissue, lymph node metastasis and TNM stage (p(p3β1expressions significantly related with tumor size. CD151 expression significantly related with ER/PR expression. The expression of CD151 was positively correlated with MMP7 and integrina3β1expression(Kappa= 0.418, Kappa =0.461). CD151 positive patients had higher rates of recurrence and metastasis than CD151negative patients(χ2=5.937 , P=0.015). Conclusions: The expressions of CD151 and integrina3β1and MMP7 were over expressed in breast carcinoma, which suggested that three proteinswere related to carcinogenesis, development, invasion and metastasis. CD151 was positively correlated with integrina3β1and MMP7 in breast cancer, which suggested that CD151 regulated integrin and MMP pathway. Joint detection of the three markers can be used to determine the biological behavior and prognosis of breast cancer.