[Development and clinical application of frozen tissue microarray in lung cancer diagnosis.].

BACKGROUND: Traditional techniques in clinical diagnostic pathology have some limitations. Here we tried to develope frozen tissue microarray as a fast, simple and economical technique. METHODS: A total of 48 dots frozen tissue microarray were constructed in this study including primary 19 lung cancer samples and 5 normal lung tissue samples from primary lung cancer. Hematoxylin-Eosin and immunohistochemical stains were performed on the sections and we assess the feasibility on morphology and genetic expression. RESULTS: The cores on frozen tissue microarray of OCT block were regularly arranged and had no shift and distort. After HE-stained, morphology of the cores was well preserved. They were answered for the request of research.Immunohistochemistry of array slides: we performed immunohistochemistry on the tumor tissue microarray with antibodies for EMA. EMA staining is uniform across the sample and gives the expected cytochylema-associated staining. There is no background staining. We compared the staining of immunohistochemistry on section of frozen tissue microarray to the results on normal slides. The results showed the accord rates were 94.7% (18/19), and there were no significant differences between them each other (P >0.05). CONCLUSIONS: The frozen tissue microarray is feasible. HE and immunohistochemical stains showing this methodology will be useful for morphology and immunohistochemical based protein analyses.