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2007•Applied and Environmental MicrobiologyOpen access

Evaluation of Postharvest-Processed Oysters by Using PCR-Based Most-Probable-Number Enumeration of Vibrio vulnificus Bacteria

Anita C. Wright, Victor Garrido, Georgia Debuex, Melissa Farrell-Evans, Archana A. Mudbidri, W. Steven Otwell

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Abstract

Postharvest processing (PHP) is used to reduce levels of Vibrio vulnificus in oysters, but process validation is labor-intensive and expensive. Therefore, quantitative PCR was evaluated as a rapid confirmation method for most-probable-number enumeration (QPCR-MPN) of V. vulnificus bacteria in PHP oysters. QPCR-MPN showed excellent correlation (R(2) = 0.97) with standard MPN and increased assay sensitivity and efficiency.

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What this paper is about

Postharvest processing (PHP) is used to reduce levels of Vibrio vulnificus in oysters, but process validation is labor-intensive and expensive. Therefore, quantitative PCR was evaluated as a rapid confirmation method for most-probable-number enumeration (QPCR-MPN) of V. vulnificus bacteria in PHP oysters. QPCR-MPN showed excellent correlation (R(2) = 0.97) with standard MPN and increased assay sensitivity and efficiency.

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Available abstract

Postharvest processing (PHP) is used to reduce levels of Vibrio vulnificus in oysters, but process validation is labor-intensive and expensive. Therefore, quantitative PCR was evaluated as a rapid confirmation method for most-probable-number enumeration (QPCR-MPN) of V. vulnificus bacteria in PHP oysters. QPCR-MPN showed excellent correlation (R(2) = 0.97) with standard MPN and increased assay sensitivity and efficiency.

Key concepts: Enumeration, Vibrio vulnificus, Most probable number, Postharvest, Bacteria, Biology, Microbiology, Vibrio

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