ScholarLens Back to search
1985•ElectrophoresisRequires access

Protein staining of 6 mm diameter sodium dodecyl sulfate‐polyacrylamide gels within one and a half hours

Birgit An der Lan, Colette Auzan, James V. Sullivan, Andreas Chrambach

Open publisher page 5 citations

Abstract

Abstract A device for rapid rotation of perforated gel holding tubes has been constructed by which the diffusion of sodium dodecyl sulfate (SDS) from cylindrical polyacrylamide gels can be substantially accelerated. This device allows one to stain protein bands obtained in SDS‐polyacrylamide gel electrophoresis (9 % gel concentration) within one and a half hours, using the procedure of fixation in trichloroacetic acid followed by Coomassie Brilliant Blue G‐250 staining, which requires no destaining.

About this research paper

What this paper is about

Abstract A device for rapid rotation of perforated gel holding tubes has been constructed by which the diffusion of sodium dodecyl sulfate (SDS) from cylindrical polyacrylamide gels can be substantially accelerated. This device allows one to stain protein bands obtained in SDS‐polyacrylamide gel electrophoresis (9 % gel concentration) within one and a half hours, using the procedure of fixation in trichloroacetic acid followed by Coomassie Brilliant Blue G‐250 staining, which requires no destaining.

Why it matters

OpenAlex reports 5 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Abstract A device for rapid rotation of perforated gel holding tubes has been constructed by which the diffusion of sodium dodecyl sulfate (SDS) from cylindrical polyacrylamide gels can be substantially accelerated. This device allows one to stain protein bands obtained in SDS‐polyacrylamide gel electrophoresis (9 % gel concentration) within one and a half hours, using the procedure of fixation in trichloroacetic acid followed by Coomassie Brilliant Blue G‐250 staining, which requires no destaining.

Key concepts: Sodium dodecyl sulfate, Polyacrylamide, Coomassie Brilliant Blue, Staining, Chromatography, Polyacrylamide gel electrophoresis, Trichloroacetic acid, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Protein staining of 6 mm diameter sodium dodecyl sulfate‐polyacrylamide gels within one and a half hours — Research Paper | ScholarLens