Staphylococcus aureus sensitivity changes to antibiotics under the influence of dissolved ozone

Aim: To study the sensitivity change of the clinical strains of staphylococcus aureus to antibiotics under the influence of dissolved ozone. (pO3). Methods: We investigated 20 S.aureus strains from sputum culture resistant antibiotics. A suspension was prepared from a day portion of the agar culture of each strain (1-2×10 8 /ml), which was treated with dissolved ozone (pO3 3mcg/ml) or with air (control) during 5-10-15 min. Then, the strains treated with pO3 were cultured on Muller-Hinton agar containing strains resistant antibiotics and incubated by 370C. In a day of incubation we recorded the results of antibiotic resistance change according the diameter zone of the growth inhibition round the antibiotic discs. Results: The growth inhibition zone increased from 3.8 to 25.7±2.1 mm in all cases of cultured S.aureus strains treated with pO3. And in cases with control strains the growth inhibition zone was not change. Conclusion: Staphylococcus aureus sensitivity to antibiotics restores after dissolved ozone treatment.