Pseudomonas aerugenosa sensitivity changes to antibiotics under the influence of dissolved ozone

Aim: To study the sensitivity changes of clinical strains of organisms producing beta-lactamases to antibiotics under the influence of dissolved ozone (pO3). Methods: We studied 20 Pseudomonas aerugenosa strains resistant to antibiotics of penicillin line. A suspension was prepared from the culture of each strain (1-2×10 8 /ml) and treated with dissolved ozone (pO3 2mcg/ml) during 5-10-15 min. Then, the stains treated with pO3 were cultured on Muller-Hinton agar containing a strain resistant antibiotic. The same strain that had not been treated with pO3 was also cultured on the same agar containing the same antibiotic (control). In a day after incubation by 370C we recorded the result of the diameter zone of growth inhibition round the antibiotic disc. Results: The zone of growth inhibition round the antibiotic disc increased from 0 to 21.6±3.5 mm in all cases of Pseudomonas aerugenosa strain culture. There were no growth changes in control cultures. Conclusion: The treatment of resistant strains of Pseudomonas aerugenosa with “therapeutic concentration” of dissolved ozone results in antibiotic sensitivity restoration.