Improved method for high-efficiency electrotransformation of Escherichia coli with the large BAC plasmids
Jana Nováková, Anita Izsáková, Tomáš Grivalský, Christian Ottmann, Marián Farkašovský
Abstract
Jana Nováková, Anita Izsáková, Tomáš Grivalský, Christian Ottmann, Marián Farkašovský
Abstract
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Key concepts: Electroporation, Plasmid, Transformation (genetics), Escherichia coli, Transformation efficiency, Biology, Bacterial artificial chromosome, Plasmid preparation