1990•FEMS Microbiology LettersRequires access

Genetic transformation of intact cells of Bacillus subtilis by electroporation

Patrizia Brigidi, Edda De Rossi, M.L. Bertarini, Giovanna Riccardi, D. Matteuzzi

Abstract

Plasmids DNAs were introduced by electroporation into Bacillus subtilis PB1424 as an alternative to competent-cell or protoplast transformation. The maximum electroporation efficiency was 104 transformants/μg DNA. Parameters including growth phase of cells, ionic strength of the suspending medium, concentration and size of plasmid DNAs, amplitude and duration of the pulse, were evaluated in order to determine conditions that improved transformations efficiency.

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Key concepts: Electroporation, Bacillus subtilis, Plasmid, Protoplast, Transformation (genetics), Transformation efficiency, Biology, Bacillaceae