2009•Ophthalmic ResearchRequires access

An Improved Method of Vital Staining of the Corneal Endothelium (With 1 color plate)

J. M. Ruiz, Magali Medrano, Jorge L. Alió

Abstract

We present an improved and simplified method for vital staining of the corneal endothelium, which allows high-quality images that are excellent for morphological and morphometric studies. This method is based upon the combination of trypan blue and alizarin red S. The main advantages of this technique are simplicity, rapidity and low cost.

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Available abstract

Key concepts: ALIZARIN RED, Corneal endothelium, Vital stain, Staining, Trypan blue, Endothelium, Simplicity, Ophthalmology