Morphological and vital study of lens epithelial cells using the trypan-blue and alizarin red staining

Objective:To assess the feasibility of trypan-blue and alizarin red vital staining to lens epithelial cells.Methords:Anterior lens capsules of rabbits,health adults and patients with cataracts were stained by trypan-blue and alizarin red,then observed the cellula appearance and the effectiveness of the staining on light microscope.Results:There were favourable effectiveness of the staining.Trypan-blue and alizarin red vital staining was capable of displaying the cellula appearance and distinguishing the normal and abnormal cells.Conclusion:Trypan-blue and alizarin red vital staining refer to lens epithelial cells,which was a practical methord to observe the morphology,density,area and the cellula vis vitalis.