1954The Journal of ProtozoologyRequires access

An Improved Method of Staining the External Organelles of Hypotrichs*

Harry J. Turner

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Abstract

A simple, rapid method of staining the external organelles of hypotrichous ciliates is described. Specimens are fixed and stained with Noland's combined phenol‐formaldehyde, gentian violet fixative and stain. The staining solution is displaced with iodized glycerine as soon as the external structures are stained to prevent it from coloring the cell body. Cirri and membranelles are stained jet black in sharp contrast to the clear amber background.

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A simple, rapid method of staining the external organelles of hypotrichous ciliates is described. Specimens are fixed and stained with Noland's combined phenol‐formaldehyde, gentian violet fixative and stain. The staining solution is displaced with iodized glycerine as soon as the external structures are stained to prevent it from coloring the cell body. Cirri and membranelles are stained jet black in sharp contrast to the clear amber background.

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Available abstract

A simple, rapid method of staining the external organelles of hypotrichous ciliates is described. Specimens are fixed and stained with Noland's combined phenol‐formaldehyde, gentian violet fixative and stain. The staining solution is displaced with iodized glycerine as soon as the external structures are stained to prevent it from coloring the cell body. Cirri and membranelles are stained jet black in sharp contrast to the clear amber background.

Key concepts: Fixative, Staining, Stain, Organelle, Chemistry, Biology, Biochemistry, Genetics

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